Refer to method which permit achieved you purpose of conducting experiments or other scientific procedures without the use of animals
Replacement alternative include:
Nonliving system or computer simulations
Refer to method which alleviate or minimize potential pain, suffering and distress and which enhance animal well-being.
Refinement alternative include:
Refer to the number of animals in the control and test group of the study. It is any scientific and statistical strategy that result in fewer animals being used to obtain the same amount of information
Reduction alternative include:
Rational selection of group size, e.g. pilot studies and power analysis Careful experiment design, e.g. appropriate choice of control groups Careful planning and evaluation of experiments Right choice of animal models Maximizing use of animals Proper selection of experimental conditions and parameters measured The appropriate use of statistical software